А microspore culture technique is used to produce homozygous lines of agronomic interest i а single generation. Several factors affecting the efficiency of in vitro sporophytic development of durum wheat microspores such as genotype, stress preatreatme t, density and co-cultivation of isolated microspore and induction medium were studied in order to develop а protocol for dihaploid plants production. Strong genotype dependence in in vitro response and callus/embryoid capacity was observed. Cultivated microspores developed into macrostructures only after cold pretrrгatment of the collected spikes at +4° С for 4 weeks. The best results were achie1v.ed at density 10000 microspores/ml оп the mod. Chu Medium (Chu et а!., 1990) 0.5 mg/1 ВАР+ 0.5 mg/1 2,40 + 90 g/1 maltosa with ovaries co-cultivation. А lot f macrostructures developed to embryoids, but only а few produced poorly-dlveloped shoots - most of them were alЬinos.
Study of factors affecting sporophytic development of isolated durum wheat microspores