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Chelidonium majus is an important medical plant used in traditional and folk medicine throughout the world. In the folk medicine of the Balkan countries it is widely used for its anticholera, antispasmodic, and sedative properties. C. majus extracts exhibit antiviral, antitumour, antimicrobial and anti-inflammatory effects. In our research we demonstrate the antiviral activity of a methanol extract from C. majus against PVY in tobacco plants. To our knowledge, it is the first investigation of an extract from this plant against phytopathogenic viruses.