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The species of genus Ranunculus L. (Buttercup) have a complex chemical composition, which is an important taxonomic feature. They synthesize saponins, alkaloids, flavonoids, phenolic acids, etc. The aim of the research was to study the changes in the content of the total phenols and flavonoids in different populations of Ranunculus arvensis L. (section Echinela) and Ranunculus repens L. (section Ranunculus) in three vegetation periods. It was found that the amount of the total phenolics and flavonoids remained constant over the years. There was a difference in the amount of the total phenols and flavonoids between R. arvensis L. and R. repens L., which was in accordance with the sectional grouping.