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Seeds of Ginkgo biloba treated with GA3 and/or IAA at different concentrations (1000, 1500, 2000 and 2500 ppm) attained the highest germination percentage (86.33%) when treated with GA3 at 2500 ppm, whilst seedling growth was stimulated in significantly higher extends using IAA with or without GA3. Furthermore, the efficacy of chilling was separately investigated at 5°C for 1-, 2- or 3-month periods comparing to room temperature which resulted in the highest germination % (64.44%).