2.5. Investigation of pharmacokinetics on marker active metabolite – 4-methylamino-antipyrin (4-MAA) after injection in pigs with drug form Analginvetg®, containing methamizole

4-methylamino-antipyrin (4-MAA) pharmacokinetics was investigated in 6 pigs (3 female and 3 male) after intramuscular injection of methamisole (Analginvet 50 % – injection solution) at a dose 25 mg/kg body weight. The plasma concentration of 4-methylamino-antipyrin was determined using high-performance liquid chromato-graphy with UV-detection. The blood concentration of 4-MAA was calculated with a compartimental and noncompartimental method using pharmacokinetic computer software (TopFit, V. 2.0.).