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A set of 5-6 water sampling points was established along the river Topolovska, Asenovgrad Municipality, flowing trough protected area “Chinar dere”. The sampling was seasonal, in conjunction with plant protection measures in agricultural field around “Chinar dere”. Samples were analysed using 16 parameters in accordance with Regulation N:7 of 08.08.1989 on quality norms of surface water. The amounts of nitrogen (N-NO2 and N-NH4–), phosphates (P043–) and phosphorus (total PO43–), both in two times of sampling can be explained with unregulated storage of animal manure and domestic waste along the river. The runoff from these piles and from arable fields flows into the river Topolovska.