The high quality in production of apple trees requests purity of the matter. That lead to the introduction of contemporary and effective production method of virus free trees – in vitro reproducing. Different methods of in vitro reproducing are investigated. The goal is to find out an effective system of tree production. One of them is the use on somatic tissue, more precisely – the leaf part. The specialized literature emphasizes on the contents of the used medium in leaf part regeneration of some fruity kinds and rootstocks.