The fungicides imazalil and carbendazim were used in the in vitro studies. Imazalil was used at 5, 10 and 20 mg/l concentrations for the rootstock CAB-6P and 2.5, 5 and 10 mg/l for the cherry rootstock Gisela 6. The fungicides were applied alone or in combination with 0.5, l and 2 mg/l benzyl-adenine (BA). Carbendazim was applied at 0.5 and 1 mg/l and combined with BA in all the tested rootstocks. The maximum number and length per shoot were 15,67 and 154,33 mm in the rootstock CAB-6P and was produced with 5 mg/l imazalil plus 1 mg/l BA. The respective numbers for Gisela 6 were 13,4 and 198 mm, in the precence of 5 mg/l imazalil plus 0.5 mg/l BA. Different ratio of carbendazim / BA concentration for each tested rootstock affected differently the number of produced shoots. Both fungicides showed a cytokinin like – activity only in the presence of BA. The efficiency of the two fungicides in combination with BA concerning shoot proliferation followed the order CAB-6P > Gisela 6.