During the period 2012-2014 23 Cucurbita pepo L. accessions, collected during expeditions in the country, were studied at IPGR Sadovo. The plant genetic resources were assessed after the Uniform Classificator (1980), consistent with the ECPGR Minimum Descriptors for Cucurbita ssp. (2008). The resulting databases were processed by applications of the R. Variability programming language for the following quantitative traits of the fruit: stalk length (cm), fruit length (cm), fruit diameter (cm), fruit shape index, locule number, skin thickness (cm), flesh thickness (cm), fruit weight (kg), and cluster analysis. The forementioned showed availability of genetic diversity in the collection. The genotypes suitable as initial material in future breeding-improvement programs were selected as a result of the comparative analysis. The local accessions with catalogue numbers 80E7437, 81E7551 and 81Е7553 were characterized by the best complex of economic qualities. All genotypes included in the study are stored at the National Gene Pool.